Journal: Leukemia

Article Title: Guidelines for the characterization and publication of human malignant hematopoietic cell lines.

doi: 10.1038/sj.leu.2401428

Figure Lengend Snippet: Figure 1 Total numbers of published cell lines according to differ- ent cell line types from 1965 to 1998 (literature summary). The types of cell lines are explained in Table 2. In this graph are not included: Burkitt lymphoma cell lines, HTLV-I + ATL cell lines and EBV + B- LCLs. Shown are only the numbers for well-characterized cell lines. A considerable number of cell lines have been reported that are only incompletely described: B cell precursor, 27; B cell, 43; plasma cell, 38; immature T cell, 6; NK cell, 2; ALCL, 5; myelocytic, 18; mono- cytic, 4; erythroid–megakaryocytic, 1. A third cohort of cell lines is not characterized at all; the number of these uncharacterized cell lines is not known. ALCL, anaplastic large cell lymphoma; ATL, adult T cell leukemia; B-LCL, B lymphoblastoid cell line; EBV, Epstein–Barr virus; HTLV, human T cell leukemia virus; NK, natural killer.

Article Snippet: ALCL cell line SU-DHL-1 Dendritic lymphoid? mono-myeloid? dendritic cell lined —d Modified after Refs 1 and 4. aThe best known cell lines for each of these categories are often the ‘oldest’ cell lines (nearly all are available from cell line banks DSMZ or ATCC); see Ref. 1 for a historical perspective. bIt is often difficult to assign cell lines to either the erythrocytic or megakaryocytic cell lineage as most of these cell lines express features of both lineages, eg (hemo)globin, specific transcription factors, surface antigens, differentiation potential, etc; thus, it is preferable to use the term ‘erythrocytic–megakaryocytic cell line’. cSee Refs 13 and 14 for reviews on this controversial cell type. dAt present, no continuous human dendritic cell line has been published; see Refs 15 and 16 for reviews on this controversial cell type. genes.11 It appears to be 10- to 100-fold easier to establish an EBV + B-LCL from a patient with leukemia than a neoplastic leukemia cell line.12 The clear distinction between a MH cell line and an EBV + B-LCL is particularly relevant for cell lines established from patients with mature B cell malignancies such as chronic lymphocytic leukemias, hairy cell leukemia, B cell non-Hodgkin’s lymphomas, myeloma and other malignancies.

Techniques: Clinical Proteomics, Virus

Journal: Haematologica

Article Title: Histone acetylation and DNA demethylation of T cells result in an anaplastic large cell lymphoma-like phenotype

doi: 10.3324/haematol.2011.054619

Figure Lengend Snippet: Expression of RYBP, TCF1, LEF1 and GATA3 in 15 cases of primary anaplastic large cell lymphoma (ALCL).

Article Snippet: Differentially expressed genes in T-cell and anaplastic large cell lymphoma cell lines To establish a gene expression signature which characteristically describes the cell type-specific differences between the T-cell lines (CCRF-CEM, Jurkat, MOLT-3 and MOLT-4) and the ALCL cell lines (FE-PD, JB6, Karpas 299 and SU-DHL-1) we compared their gene expression profiles (untreated cells).

Techniques: Expressing

Journal: Haematologica

Article Title: Histone acetylation and DNA demethylation of T cells result in an anaplastic large cell lymphoma-like phenotype

doi: 10.3324/haematol.2011.054619

Figure Lengend Snippet: The ID2 promoter region is demethylated in 5-aza-dC/TSA-treated T cells and in tumor cells of primary ALCL. The methylation status of the ID2 promoter was analyzed by bisulfite sequencing. The sequenced region in the ID2 promoter contained six CpG dinucleotides. Clones were sequenced from 5-aza-dC/TSA-treated and untreated Jurkat and MOLT-3 cells and four primary ALCL cases. Every row represents a single PCR clone.

Article Snippet: Differentially expressed genes in T-cell and anaplastic large cell lymphoma cell lines To establish a gene expression signature which characteristically describes the cell type-specific differences between the T-cell lines (CCRF-CEM, Jurkat, MOLT-3 and MOLT-4) and the ALCL cell lines (FE-PD, JB6, Karpas 299 and SU-DHL-1) we compared their gene expression profiles (untreated cells).

Techniques: Methylation, Methylation Sequencing, Clone Assay

Journal: Haematologica

Article Title: Histone acetylation and DNA demethylation of T cells result in an anaplastic large cell lymphoma-like phenotype

doi: 10.3324/haematol.2011.054619

Figure Lengend Snippet: (A) Promoter H3K27 trimethylation status of T-cell signaling pathway genes and T-cell transcription factor genes after ChIP and quantification by real-time DNA-PCR in T- and ALCL cell lines. β-ACTIN was chosen as the endogenous reference and the input was calculated relative to the ChIP input control. (B) Immunohistochemistry for GATA3 (original magnification, 400×), LEF1 and TCF1 (original magnification 200×) in primary ALCL cases. All three T-cell transcription factors were strongly expressed in the nuclei of the reactive T cells whereas the tumor cells displayed no or only faint expression.

Article Snippet: Differentially expressed genes in T-cell and anaplastic large cell lymphoma cell lines To establish a gene expression signature which characteristically describes the cell type-specific differences between the T-cell lines (CCRF-CEM, Jurkat, MOLT-3 and MOLT-4) and the ALCL cell lines (FE-PD, JB6, Karpas 299 and SU-DHL-1) we compared their gene expression profiles (untreated cells).

Techniques: Control, Immunohistochemistry, Expressing